A revised and updated checklist of Monogononta rotifers from Argentina

We provide here a checklist of species of Monogononta rotifers from lentic and lotic environments in Argentina, 25 years after the initial catalogue compiled by Susana B. José de Paggi. This new inventory now includes the reports on rotifers documented in 93 studies produced after 1990. The majority of the investigations were carried out in three of the 24 Argentine provinces. In addition, the presence of 13 species in samples from three water bodies within Buenos Aires province are now cited here for the first time in Argentina. In this updated checklist, a total of 351 species are catalogued, the majority being representatives of the Lecanidae, Brachionidae, and Lepadellidae.Instituto de Limnología "Raúl A. Ringuelet

A revised and updated checklist of Monogononta rotifers from Argentina

We provide here a checklist of species of Monogononta rotifers from lentic and lotic environments in Argentina, 25 years after the initial catalogue compiled by Susana B. José de Paggi. This new inventory now includes the reports on rotifers documented in 93 studies produced after 1990. The majority of the investigations were carried out in three of the 24 Argentine provinces. In addition, the presence of 13 species in samples from three water bodies within Buenos Aires province are now cited here for the first time in Argentina. In this updated checklist, a total of 351 species are catalogued, the majority being representatives of the Lecanidae, Brachionidae, and Lepadellidae.Instituto de Limnología "Raúl A. Ringuelet

A quick and effective estimation of algal density by turbidimetry developed with Chlorella vulgaris cultures

El uso de Chlorella vulgaris Beijerinck como alimento para el zooplancton implica la necesidad de optimizar el cultivo de algas para mantener su crecimiento en el tiempo. En este trabajo se desarrolló un método que relaciona la densidad del cultivo con la turbidez para estimar la biomasa algal. Esta técnica se ha mejorado mediante la aplicación del análisis digital para el recuento de las algas que promueve la exactitud y reduce el disturbio en el cultivo, con obtención rápida y fácil de resultados repetibles. Se realizaron dos cultivos de C. vulgaris en recipientes de 3 L con aireación e iluminación continua (50 µmol fotones m–2 ·s–1 a 660 nm), alcanzando 214 y 280 NTU, respectivamente. El recuento de las muestras se realizó por medio de imágenes digitales tomadas con un microscopio invertido. Se aplicaron dos técnicas para el recuento de las alícuotas: la sedimentación directa y la sedimentación con homogeneización previa. Con el fin de comparar el ajuste de ambos métodos de sedimentación, las fotografías fueron tomadas en los sectores central, medio y periférico de la cámara de sedimentación. Para ambas técnicas se contaron un mínimo de 17 individuos imagen–1 y un máximo de 404 individuos imagen–1, con un tiempo promedio de un minuto por imagen. A niveles bajos de turbidez (< 40 NTU) la dispersión de los datos fue similar entre ambas técnicas (rango error: 16-60 %). Para niveles superiores de turbidez, en la técnica de sedimentación directa se observó un mayor rango de error (31-50 %) mientras que en la técnica con homogenización previa osciló entre 5 y 13 %. El análisis de regresión evidenció un bajo ajuste de los datos (67 %), que en la sedimentación sin homogeneización responde a un patrón reiterado de aumento de densidad algal desde la periferia hacia el centro de la cámara de sedimentación. La inclusión de una homogeneización previa promueve un mejor ajuste del modelo (99 %) y determina un incremento en la consistencia del método. Con los resultados obtenidos se demuestra que la técnica turbidimétrica desarrollada puede ser utilizada con éxito en cultivos de especies de algas cuyas formas geométricas sean reconocidas por el procesador de imágenes.The use of Chlorella vulgaris Beijerinck as a food source for zooplankton requires the optimization of algal-culture conditions for prolonged growth maintenance. In this study, we developed a method that relates algal density to culture turbidity to estimate culture biomass. This method was improved by applying digital analysis for algal counting, which promotes accuracy, low culture disturbance, easy repetition and the rapid acquisition of results. Two 3-L cultures of C. vulgaris, maintained for two weeks with continuous lighting (eight light-emitting diodes at 50 µmol photons m–2 ·s–1, at 660 nm) and aerators to prevent algal sedimentation, reached turbidities of 214 and 280 NTUs, respectively. Sample counting was performed using digital images obtained with an inverted microscope. Aliquot sedimentation was compared with or without previous homogenization through photographs taken in the central, middle, and peripheral sectors of the Utermöhl settling chambers. For each procedure, we counted between 17 and 404 individuals image–1, requiring, on average, one minute image–1. At low turbidity (< 40 NTU), the data dispersion was similar between the two protocols (error range, 16 to 60 %); at higher turbidity, the direct sedimentation alone gave a larger error (31-50 %) than with prior homogenization (5-13 %). Regression analysis at low data fit (67 %) suggested that the sedimentation heterogeneity of non-homogenized samples corresponded to a pattern of settled algae having increasing density from the periphery to the centre of the chamber, but with homogenization, a better model fitting (99 %) resulted, contributing to greater consistency with that procedure. We consider that this turbidometric protocol could be used successfully with cultures of algae that have geometrical shapes recognizable by the image software.Fil: Ferrando, Noelia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Benitez, Hernan Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Gabellone, Nestor Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Claps, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Altamirano, Pablo Rodrigo. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentin

Influence of temperature and conductivity on the life-history characteristics of a pampean strain of Brachionus plicatilis

In the present work, we provide the first approach about the life-history of Brachionus plicatilis in South America. We tested with laboratory experiments the response of the pampean strain of B. plicatilis for two of its main stressors (conductivity and temperature). We evaluated the effects of eight conductivity values from 1 to 17 mS.cm-1 and two temperatures (15 and 25 °C) to compare its abundance with those obtained in the pampean lotic and lentic environments, where this rotifer is frequent or dominant. The results demonstrated that the increase in population-growth rate and the peak of abundance occurred at the highest temperature and at medium conductivity. Minimum values were obtained at the lowest temperature and conductivities analyzed, but the final density attained was nevertheless similar to those recorded in the pampean environments at the optimum conductivity and during the spring and summer seasons. Males, mictic females, and resting eggs were observed at the minimum and maximum conductivities, revealing the strategy of this species for maintaining dominance in environments with fluctuating salinity. The experiments also indicated the possible behavior of this relevant member of the zooplankton community within a scenario of increasing temperature and salinity related to the climate changes occurring in the pampean region.Instituto de Limnología "Raúl A. Ringuelet

Influence of temperature and conductivity on the life-history characteristics of a pampean strain of Brachionus plicatilis

In the present work, we provide the first approach about the life-history of Brachionus plicatilis in South America. We tested with laboratory experiments the response of the pampean strain of B. plicatilis for two of its main stressors (conductivity and temperature). We evaluated the effects of eight conductivity values from 1 to 17 mS.cm-1 and two temperatures (15 and 25 °C) to compare its abundance with those obtained in the pampean lotic and lentic environments, where this rotifer is frequent or dominant. The results demonstrated that the increase in population-growth rate and the peak of abundance occurred at the highest temperature and at medium conductivity. Minimum values were obtained at the lowest temperature and conductivities analyzed, but the final density attained was nevertheless similar to those recorded in the pampean environments at the optimum conductivity and during the spring and summer seasons. Males, mictic females, and resting eggs were observed at the minimum and maximum conductivities, revealing the strategy of this species for maintaining dominance in environments with fluctuating salinity. The experiments also indicated the possible behavior of this relevant member of the zooplankton community within a scenario of increasing temperature and salinity related to the climate changes occurring in the pampean region.Instituto de Limnología "Raúl A. Ringuelet

Influence of temperature and conductivity on the life-history characteristics of a pampean strain of Brachionus plicatilis

In the present work, we provide the first approach about the life-history of Brachionus plicatilis in South America. We tested with laboratory experiments the response of the pampean strain of B. plicatilis for two of its main stressors (conductivity and temperature). We evaluated the effects of eight conductivity values from 1 to 17 mS.cm-1 and two temperatures (15 and 25 °C) to compare its abundance with those obtained in the pampean lotic and lentic environments, where this rotifer is frequent or dominant. The results demonstrated that the increase in population-growth rate and the peak of abundance occurred at the highest temperature and at medium conductivity. Minimum values were obtained at the lowest temperature and conductivities analyzed, but the final density attained was nevertheless similar to those recorded in the pampean environments at the optimum conductivity and during the spring and summer seasons. Males, mictic females, and resting eggs were observed at the minimum and maximum conductivities, revealing the strategy of this species for maintaining dominance in environments with fluctuating salinity. The experiments also indicated the possible behavior of this relevant member of the zooplankton community within a scenario of increasing temperature and salinity related to the climate changes occurring in the pampean region.Instituto de Limnología "Raúl A. Ringuelet

Primera mención del caracol invasor Melanoides tuberculatus (Müller) (Gastropoda: Prosobranchia: Thiaridae) para la Cuenca del Río Iguazú, Argentina – Brasil

In this work is reported the first record of Melanoides tuberculatus in the Iguazú River basin (Argentina-Brazil). This species is native to southern Asia and was anthropogenically introduced to America in the 1960s decade. Sixteen specimens (one adult and 15 juvenile) of M. tuberculatus were recorded at only one site, the rapids of Iguazú River at Isla San Martín, Iguazú National Park, Argentina, in June 2005. This finding of M. tuberculatus specimens suggests the presence of a stable population that would represent a threat to the native mollusk fauna, given that this species promotes replacement and impoverishment of mollusk diversity.En este trabajo se reporta el primer registro de Melanoides tuberculatus en la cuenca del río Iguazú (Argentina-Brasil). Esta especie es nativa del sudeste asiático y fue introducida por el hombre en el continente americano en la década de 1960. Dieciséis ejemplares (un adulto y 15 juveniles) de M. tuberculatus fueron colectados en junio de 2005 en un solo sitio del Parque Nacional Iguazú (Argentina), correspondiente a los rápidos de la Isla San Martín. Este hallazgo sugiere la presencia de una población estable que podría representar una amenaza a la fauna de moluscos nativa, dado que esta especie provoca un reemplazo y empobrecimiento de la diversidad de moluscos.Facultad de Ciencias Naturales y Muse

Primera mención del caracol invasor Melanoides tuberculatus (Müller) (Gastropoda: Prosobranchia: Thiaridae) para la Cuenca del Río Iguazú, Argentina – Brasil

In this work is reported the first record of Melanoides tuberculatus in the Iguazú River basin (Argentina-Brazil). This species is native to southern Asia and was anthropogenically introduced to America in the 1960s decade. Sixteen specimens (one adult and 15 juvenile) of M. tuberculatus were recorded at only one site, the rapids of Iguazú River at Isla San Martín, Iguazú National Park, Argentina, in June 2005. This finding of M. tuberculatus specimens suggests the presence of a stable population that would represent a threat to the native mollusk fauna, given that this species promotes replacement and impoverishment of mollusk diversity.En este trabajo se reporta el primer registro de Melanoides tuberculatus en la cuenca del río Iguazú (Argentina-Brasil). Esta especie es nativa del sudeste asiático y fue introducida por el hombre en el continente americano en la década de 1960. Dieciséis ejemplares (un adulto y 15 juveniles) de M. tuberculatus fueron colectados en junio de 2005 en un solo sitio del Parque Nacional Iguazú (Argentina), correspondiente a los rápidos de la Isla San Martín. Este hallazgo sugiere la presencia de una población estable que podría representar una amenaza a la fauna de moluscos nativa, dado que esta especie provoca un reemplazo y empobrecimiento de la diversidad de moluscos.Facultad de Ciencias Naturales y Muse

Una estimación rápida y eficaz de la densidad algal por medio de un método turbidimétrico desarrollado con cultivos de Chlorella vulgaris

The use of Chlorella vulgaris Beijerinck as a food source for zooplankton requires the optimization of algal-culture conditions for prolonged growth maintenance. In this study, we developed a method that relates algal density to culture turbidity to estimate culture biomass. This method was improved by applying digital analysis for algal counting, which promotes accuracy, low culture disturbance, easy repetition and the rapid acquisition of results. Two 3-L cultures of C. vulgaris, maintained for two weeks with continuous lighting (eight light-emitting diodes at 50 μmol photons m–2 · s–1, at 660 nm) and aerators to prevent algal sedimentation, reached turbidities of 214 and 280 NTUs, respectively. Sample counting was performed using digital images obtained with an inverted microscope. Aliquot sedimentation was compared with or without previous homogenization through photographs taken in the central, middle, and peripheral sectors of the Utermöhl settling chambers. For each procedure, we counted between 17 and 404 individuals image–1, requiring, on average, one minute image–1. At low turbidity (< 40 NTU), the data dispersion was similar between the two protocols (error range, 16 to 60%); at higher turbidity, the direct sedimentation alone gave a larger error (31-50 %) than with prior homogenization (5-13 %). Regression analysis at low data fit (67 %) suggested that the sedimentation heterogeneity of non-homogenized samples corresponded to a pattern of settled algae having increasing density from the periphery to the centre of the chamber, but with homogenization, a better model fitting (99 %) resulted, contributing to greater consistency with that procedure. We consider that this turbidometric protocol could be used successfully with cultures of algae that have geometrical shapes recognizable by the image software.El uso de Chlorella vulgaris Beijerinck como alimento para el zooplancton implica la necesidad de optimizar el cultivo de algas para mantener su crecimiento en el tiempo. En este trabajo se desarrolló un método que relaciona la densidad del cultivo con la turbidez para estimar la biomasa algal. Esta técnica se ha mejorado mediante la aplicación del análisis digital para el recuento de las algas que promueve la exactitud y reduce el disturbio en el cultivo, con obtención rápida y fácil de resultados repetibles. Se realizaron dos cultivos de C. vulgaris en recipientes de 3 L con aireación e iluminación continua (50 μmol fotones m–2 · s–1 a 660 nm), alcanzando 214 y 280 NTU, respectivamente. El recuento de las muestras se realizó por medio de imágenes digitales tomadas con un microscopio invertido. Se aplicaron dos técnicas para el recuento de las alícuotas: la sedimentación directa y la sedimentación con homogeneización previa. Con el fin de comparar el ajuste de ambos métodos de sedimentación, las fotografías fueron tomadas en los sectores central, medio y periférico de la cámara de sedimentación. Para ambas técnicas se contaron un mínimo de 17 individuos imagen–1 y un máximo de 404 individuos imagen–1, con un tiempo promedio de un minuto por imagen. A niveles bajos de turbidez (< 40 NTU) la dispersión de los datos fue similar entre ambas técnicas (rango error: 16-60 %). Para niveles superiores de turbidez, en la técnica de sedimentación directa se observó un mayor rango de error (31-50 %) mientras que en la técnica con homogenización previa osciló entre 5 y 13%. El análisis de regresión evidenció un bajo ajuste de los datos (67%), que en la sedimentación sin homogeneización responde a un patrón reiterado de aumento de densidad algal desde la periferia hacia el centro de la cámara de sedimentación. La inclusión de una homogeneización previa promueve un mejor ajuste del modelo (99%) y determina un incremento en la consistencia del método. Con los resultados obtenidos se demuestra que la técnica turbidimétrica desarrollada puede ser utilizada con éxito en cultivos de especies de algas cuyas formas geométricas sean reconocidas por el procesador de imágenes.Instituto de Limnología "Dr. Raúl A. Ringuelet"Facultad de Ciencias Agrarias y Forestale

Influence of temperature and conductivity on the life-history characteristics of a pampean strain of Brachionus plicatilis

In the present work, we provide the first approach about the life-history of Brachionus plicatilis in South America. We tested with laboratory experiments the response of the pampean strain of B. plicatilis for two of its main stressors (conductivity and temperature). We evaluated the effects of eight conductivity values from 1 to 17 mS.cm-1 and two temperatures (15 and 25 °C) to compare its abundance with those obtained in the pampean lotic and lentic environments, where this rotifer is frequent or dominant. The results demonstrated that the increase in population-growth rate and the peak of abundance occurred at the highest temperature and at medium conductivity. Minimum values were obtained at the lowest temperature and conductivities analyzed, but the final density attained was nevertheless similar to those recorded in the pampean environments at the optimum conductivity and during the spring and summer seasons. Males, mictic females, and resting eggs were observed at the minimum and maximum conductivities, revealing the strategy of this species for maintaining dominance in environments with fluctuating salinity. The experiments also indicated the possible behavior of this relevant member of the zooplankton community within a scenario of increasing temperature and salinity related to the climate changes occurring in the pampean region